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mirna rapid extraction kits  (Bioteke Corporation)

 
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    Bioteke Corporation mirna rapid extraction kits
    Mirna Rapid Extraction Kits, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirnas+rapid+extraction+kit/mirna+extraction+kit/pm37080368-93-31-35
    Average 90 stars, based on 1 article reviews
    mirna rapid extraction kits - by Bioz Stars, 2026-09
    90/100 stars

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    Extraction:

    Article Title: <p>MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma</p>
    Article Snippet: The first-strand cDNAwas synthesized by Reverse Transcription System (Thermo Fisher Scientific, Waltham, MA, USA). .. MiRNAs Rapid Extraction Kit (BioTeke, Beijing, China) was employed to extract total miRNAs. .. RT-qPCR was performed using SYBR® Premix Ex TaqTM (TaKaRa, Dalian, China) in ABI7500 sequence detector (Applied Biosystems, Foster City, CA, USA).

    Article Title: Sp1-driven up-regulation of miR-19a decreases RHOB and promotes pancreatic cancer
    Article Snippet: .. MiRNAs were extracted from pancreatic cancer tissues, and ASPC-1 cells with miRNAs rapid extraction kit (BioTeke), and Omega E.Z.N.A miRNA kit, respectively. .. Detection was carried out by quantitative RT-PCR with Hairpin-it TM miRNA PCR quantitation kit (GenePharma) and specific primers for miR-19a (GenePharma) respectively, according to the manufacturer's instructions.

    Article Title: MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma
    Article Snippet: The first-strand cDNA was synthesized by Reverse Transcription System (Thermo Fisher Scientific, Waltham, MA, USA). .. MiRNAs Rapid Extraction Kit (BioTeke, Beijing, China) was employed to extract total miRNAs. .. RT-qPCR was performed using SYBR® Premix Ex TaqTM (TaKaRa, Dalian, China) in ABI7500 sequence detector (Applied Biosystems, Foster City, CA, USA).



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    (A, B) RT-qPCR results showed that miR-1268b could inhibit the expression of PIK3CA, AKT2, AKT3 and Bcl2 in MDA-MB-453 (A) and SKBR3 (B) ( * p <0.05, ** p <0.01, *** p<0.001). (C, D, E, F) Western blot assay further showed that <t>miRNA-1268b</t> reduced the protein expression of PIK3CA, AKT and Bcl2 in MDA-MB-453 (C, D) and SKBR3 (E, F) ( * p < 0.05). (G) The schematic diagram showed the potential functional mechanism of miR-1268b to induce chemosensitivity in breast cancer. MiR-1268b could repress the PI3K-AKT signaling pathway by targeting ERBB2 and inhibit the anti-apoptosis protein Bcl2.
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    mirna rapid extraction kit (spin-column) rp5301  (Bioteke Corporation)
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    (A, B) RT-qPCR results showed that miR-1268b could inhibit the expression of PIK3CA, AKT2, AKT3 and Bcl2 in MDA-MB-453 (A) and SKBR3 (B) ( * p <0.05, ** p <0.01, *** p<0.001). (C, D, E, F) Western blot assay further showed that <t>miRNA-1268b</t> reduced the protein expression of PIK3CA, AKT and Bcl2 in MDA-MB-453 (C, D) and SKBR3 (E, F) ( * p < 0.05). (G) The schematic diagram showed the potential functional mechanism of miR-1268b to induce chemosensitivity in breast cancer. MiR-1268b could repress the PI3K-AKT signaling pathway by targeting ERBB2 and inhibit the anti-apoptosis protein Bcl2.
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    (A, B) RT-qPCR results showed that miR-1268b could inhibit the expression of PIK3CA, AKT2, AKT3 and Bcl2 in MDA-MB-453 (A) and SKBR3 (B) ( * p <0.05, ** p <0.01, *** p<0.001). (C, D, E, F) Western blot assay further showed that miRNA-1268b reduced the protein expression of PIK3CA, AKT and Bcl2 in MDA-MB-453 (C, D) and SKBR3 (E, F) ( * p < 0.05). (G) The schematic diagram showed the potential functional mechanism of miR-1268b to induce chemosensitivity in breast cancer. MiR-1268b could repress the PI3K-AKT signaling pathway by targeting ERBB2 and inhibit the anti-apoptosis protein Bcl2.

    Journal: Oncotarget

    Article Title: MiR-1268b confers chemosensitivity in breast cancer by targeting ERBB2-mediated PI3K-AKT pathway

    doi: 10.18632/oncotarget.20099

    Figure Lengend Snippet: (A, B) RT-qPCR results showed that miR-1268b could inhibit the expression of PIK3CA, AKT2, AKT3 and Bcl2 in MDA-MB-453 (A) and SKBR3 (B) ( * p <0.05, ** p <0.01, *** p<0.001). (C, D, E, F) Western blot assay further showed that miRNA-1268b reduced the protein expression of PIK3CA, AKT and Bcl2 in MDA-MB-453 (C, D) and SKBR3 (E, F) ( * p < 0.05). (G) The schematic diagram showed the potential functional mechanism of miR-1268b to induce chemosensitivity in breast cancer. MiR-1268b could repress the PI3K-AKT signaling pathway by targeting ERBB2 and inhibit the anti-apoptosis protein Bcl2.

    Article Snippet: After cut into 4-μm slices, dewaxed, rehydrated and stained with hematoxylin, FFPE tissues were disposed for microRNAs extraction using a miRNA rapid extraction kit (Bioteke, Beijing, China).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Functional Assay